Chromatogram deconvolution

Savitzky-Golay

This method uses the Savitzky-Golay polynomial (A. Savitzky and M. J. E. Golay, Anal. Chem., 36, 1627 (1964)) to calculate the smoothed second-derivative of the chromatogram's intensities. The following figure (left) presents the shape of (a) a Gaussian peak, (b) the first derivative, and (c) the second derivative. The figure on the right side shows how the signal (blue line) may be divided into individual chromatographic peaks by observing the second derivative.

Savitzky-Golay filter Savitzky-Golay result

Method parameters

Min peak height
Minimum acceptable peak height (absolute intensity)
Peak duration range
Range of acceptable peak durations
Derivative threshold level
Minimum acceptable intensity in the second derivative for peak recognition

Savitzky-Golay example