Chromatogram deconvolution

Local minimum search

This method is suitable for chromatograms with little noise and nice peak shapes. It searches for local minima in the chromatogram and separates individual peaks at these minimal points. Individual peaks are recognized if they fulfill the height and duration requirements. The Chromatographic threshold parameter can be used if the chromatogram contains some background noise.

Method parameters

Chromatographic threshold
Threshold for removing noise. The algorithm finds the intensity such that the specified percentage of the chromatogram's data points are below that intensity; all such data points are removed.
Search minimum in RT range
A local minimum is considered to be the point that separates two adjacent peaks if it is minimal in the specified retention time range
Minimum relative height
Minimum height of a peak relative to the chromatogram's highest data point
Minimum absolute height
Minimum absolute height of a peak for it to be recognized
Min ratio of peak top/edge
Minimum ratio between a peak's top intensity and side (lowest) data points. This parameter helps to reduce the detection of false peaks in cases where the chromatogram is not smooth.
Peak duration range
Range of acceptable peak durations

Local minimum search example