Question 0 - Me

Question 1 - Proficiency

Question 2

Question 3 - Loading Custom Data - IGV

Download the bigwig file located here and load it into IGV. Identify the peak value in the given genomic windows to the nearest integer:

  • chr2:9,725,036-9,725,661
  • chr2:10,268,923-10,269,078
  • chr2:11,242,850-11,243,005
  • chr7:48,672,185-48,672,594
  • chr7:132,719,406-132,719,456

Answer below:

Question 4 - Loading Custom Data - UCSC

UCSC cannot directly accept file uploads so copy this address "http://hgdownload.cse.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeHaibRnaSeq/wgEncodeHaibRnaSeqJurkatRawRep2.bigWig" and load it into the UCSC genome browser by going here and pasting it into the first box.
View the track by going here. Leave DEFAULT settings on.

Identify the peak value in the given genomic windows to the nearest integer:

  • chr21:19,902,930-20,678,082
  • chr21:44,395,072-44,958,819
  • chr20:18,536,476-19,031,716
  • chr20:32,184,170-32,836,757
  • chr20:34,263,756-34,368,858

Answer below:

Question 5 - Loading Custom Data - PBrowse

Download the bigwig file located here and load it into the genome browser.

Select a track and use Shift+Uparrow/Downarrow to make it bigger/smaller
Identify the peak value in the given genomic windows to the nearest integer:

  • 11:25,609,719..25,610,653
  • 8:30,432,430..30,441,578
  • 8:27,689,770..27,691,661
  • 3:23,175,361..23,177,254
  • 3:25,641,778..25,643,671

Answer below:

Question 6 - Identify gene positions - UCSC

Using the UCSC browser with DEFAULT SETTINGS, identify and record the positions of the following REFSEQ genes, no extra data files are necessary:

  • RALY
  • BPI
  • SNTA1
  • LINC00657
  • AAR2

Answer below:

Question 7 - Identify gene positions - IGV

Using the genome browser, identify and record the positions of the following genes:

  • AUTS2
  • TES
  • EVX1
  • GPR141
  • ING3

Answer below:

Question 8 - Identify gene positions - PBrowse

Using the genome browser, identify and record the positions of the following genes.

CLICK on a gene in the view to see FULL DETAILS:

  • LUZP2
  • HTATIP2
  • SOX12
  • CENPB
  • HAO1

Answer below: