The retention time normalizer attempts to reduce the deviation of retention times between peak lists, by searching for common peaks in these peak lists and using them as normalization standards. The method requires multiple peak lists of different samples, processed by Deconvolution, but prior to alignment. Ions which are present in all given peak lists (according to given m/z and RT tolerance and minimum intensity) in exactly one instance are considered as standards. Retention times of the standards are then averaged and equalized in all samples, and retention times of all other peaks are adjusted according to the retention times of neighboring standard peaks.
The folowing screenshot shows two peak lists (top) normalized by the Retention time normalizer (bottom) with the following parameters: m/z tolerance = 0.1, RT tolerance = 1:00, Minimum standard intensity = 1E6. The red color shows which peaks were selected as standards. The retention time of standards was averaged, and retention time of other peaks was adjusted accordingly (blue color).