Chromatogram deconvolution

Noise amplitude

This method is suitable for chromatograms with significant background noise of varying intensities. It works in a similar way to the Baseline cut-off method, but sets the baseline level individually for each chromatogram, depending on the amplitude of signal noise.

The baseline level is calculated as follows:

  1. The intensity range of the chromatogram is divided into bins of the user-specified size (the Noise amplitude parameter)
  2. The bin with the highest number of data points is found. This bin represents the intensity level of the noise signal.
  3. The baseline level is set to the intensity of the bin with the most data points

Noise analysis example Noise analysis example (zoomed)

Method parameters

Min peak height
Minimum acceptable height (intensity) for a chromatographic peak
Peak duration range
Range of acceptable peak durations
Amplitude of noise
This value is the intensity amplitude of the signal in the noise region

Noise amplitude example