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...made Pythony

The Gibthon Construct Designer facilitates the design of primers to create overlaps in your DNA. It will optimise them for length and melting temperature, and warn you of any potential selfpriming or mispriming.

How does the GCD work?

Gibson Assembly...

Gibson Assembly is a revolutionary new techinque described by Daniel Gibson in 2009 that allows for seamless joining of an arbitrary number of sequences.

How does Gibson Assembly work?

About

Gibthon was started for the 2010 Cambridge iGEM Team as a companion to our RFC on Gibson Assembly.

It is written and currently hosted by Bill Collins using the Django Framework, and makes extensive use of mfold, BioPython and jQuery. The source code will be relased when this site goes live.

The project has been made possible thanks to the invaluable advice and academic and fiscal support of Jim Ajioka, Jim Haseloff, and Gos Micklem.

Ligation Calculator

The Ligation Calculator is a simple tool for calculating reactant concentrations for DNA ligation

Ligation Calculator

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