Overview

Numbers next to primers indicate number of warnings for that primer.

Click on the primers to see details.

{% include "gibson/construct_preview.html" %}

Primer Summary

{% for primer in construct.primer.all %} {% endfor %}
Name Length (bp) Tm anneal (°C) Tm full (°C)
{{ primer.name }} {{ primer.length }} {{ primer.stick.tm|floatformat:0 }} {{ primer.tm|floatformat:0 }}

PCR Particulars

Fill in the details for all of your primers to download protocol information

Don't forget to save your changes!

Warning! Some of your components have a volume of less than 1μl. Consider diluting them before adding to the mix.
Stock
{% for cf in construct.cf.all %}
{{ cf.fragment.name }}Top μM
Template ng/μlBottom μM
 
{% endfor %}
 
Repeats
Volume each μl
Error margin %
ReagentStockDesired
Buffer X X
dNTPs mM mM
Enzyme U/μl U
Primer μM
Template ng

If you see an orange box, it means that you will need to pipette less than 1μl of something. You are advised to dilute before adding to the mix.