2019-04-24 v1.3.1
- Updated instructions
- Updated inputs to allow linebreaks
2019-03-03 v1.3.0
- Added Ensembl support
- Updated Homology Design instructions (v2.2)
- Fixed HTML formatting
- Updated GTagHD/GeneWeld information
- Moved information from "Submit Job" tab to "Instructions and FAQs" to streamline UI
- Updated Downloads URLs
- Added AddGene links for acquiring GeneWeld plasmids
2019-02-01 v1.2.2
- Updated CSS to match GSS homepage
2018-07-17 v1.2.1
- Fixed enzyme overhangs for custom plasmids and pPRISM
- Updated changelog HTML formatting
- Fixed padding nucleotide addition and generation to repair using genomic sequence
2018-07-16 v1.2.0
- Added pPRISM enzyme options
- Readability code fixes
- Updated instructions
- Added FAQs and Troubleshooting information
- Updated pGTag plasmid files with new header information
2018-01-27 v1.1.0
2018-01-26 v1.0.10
- Added fixes to prevent crashes on invalid microhomology length in copy/paste submissions
2018-01-25 v1.0.9
- Fixed GenBank file retrieval
- Added 'About' information
- Added Gene Sculpt Suite logo
- Miscellaneous formatting updates
2018-01-17
- Miscellaneous formatting updates
2018-01-12 v1.0.8
- Added dynamic plasmid oligonucleotide incorporation
2017-12-19 v1.0.7
- Added embedded Google Form for bug reporting
- Fixed several logos and links
2017-10-24 v1.0.6
- Resolved issue wherein GenBank accessions with both 'exon' and 'CDS' locus tags threw errors
- Modified code to avoid generating the reverse complement of the input gene sequence
2017-10-18
- Added GenBank format plasmids for download
2017-10-10 v1.0.5
- Revised instructions for clarity
- Added support for non-SpCas9 CRISPRs
- Implemented GenBank sequence retrieval and processing
- Added automatic exon repair for GenBank sequences
- Added progress bar
Known Issues:
- Some GenBank accessions will cause a crash due to a conflict between exon and cds locus tags in those files; fix in progress
2017-08-23 v1.0.4
- Fixed multiple typos in instructions and links
- Uploaded missing "Designing Oligos" pdf document
2017-08-10 v1.0.3
- Updated user interface to streamline certain options in prep and fix typos.
2017-08-01 v1.0.2
- Fixed ordering issue with non-homologous spacer and padding nucleotides in instances where the reverse complement of the guide is found in the cDNA
2017-07-11 v1.0.1
2017-07-08 v1.0.0
- Official v1 Release
- Added progress bar when querying ENSEMBL
Bug Fixes:
- Fixed time-out crashes in querying ENSEMBL database
2017-06-27 v0.0.5
- Added GTAG plasmid series for oligo integration
- Added download button for oligos
Bug Fixes:
- Fixed 3' reverse oligo restriction site
2017-04-06 v0.0.4
- Added auto-detect of species by ENSEMBL gene ID
Bug Fixes:
- Fixed form not populating/submitting on ENSEMBL gene ID selection
2017-04-05 v0.0.3
- Added validation checks on gene ID
Bug Fixes:
- Solved issue where app crashed when submitting invalid inputs
2017-04-04 v0.0.2
- Added cloning overhangs in generated oligo sequences
2017-04-03 v0.0.1
- Added ability to use ENSEMBL gene ID instead of cDNA sequence
- Added support for searching the reverse complement of the cDNA sequence
Known Issues:
- Crash to desktop when submitting invalid inputs
Next major update:
- Validation checks for ENSEMBL inputs